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MacBinary  |  1990-06-10  |  8.3 KB  |  [GCKc/GCK ]
open in: MacOS 8.1     |     Win98     |     DOS

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This file was processed as: MacBinary (archive/macBinary).

You can browse this item here: pGEM3

ConfidenceProgramDetectionMatch TypeSupport
10% dexvert MacBinary (archive/macBinary) fallback Supported
10% dexvert Jesper Olsen Module (music/jesperOlsen) magic Supported
100% file MacBinary II, Wed Dec 13 11:38:16 1989, modified Sat Jun 9 21:12:06 1990, creator 'GCK ', type 'GCKc', 7729 bytes "pGEM3" , at 0x1eb1 480 bytes resource default (weak)
99% file data default
65% TrID TTComp archive compressed (bin-2K) default (weak)
33% TrID MacBinary 2 default (weak)
0% TrID Sybase iAnywhere database files default (weak)
100% lsar MacBinary default


id metadata
keyvalue
macFileType[GCKc]
macFileCreator[GCK ]


hex view
+--------+-------------------------+-------------------------+--------+--------+
|00000000| 00 05 70 47 45 4d 33 00 | 00 00 00 00 00 00 00 00 |..pGEM3.|........|
|00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000040| 00 47 43 4b 63 47 43 4b | 20 00 00 00 00 00 00 00 |.GCKcGCK| .......|
|00000050| 00 00 00 00 00 1e 31 00 | 00 01 e0 a1 ac 2b 78 a2 |......1.|.....+x.|
|00000060| 97 4e e6 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |.N......|........|
|00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 c7 26 00 00 |........|.....&..|
|00000080| 00 00 00 03 ff ff 80 01 | 00 00 00 00 00 00 00 00 |........|........|
|00000090| 00 00 01 19 00 00 01 59 | 00 00 0b 36 00 00 0b 32 |.......Y|...6...2|
|000000a0| 47 41 41 54 41 43 41 41 | 47 43 54 54 47 43 41 54 |GAATACAA|GCTTGCAT|
|000000b0| 47 43 43 54 47 43 41 47 | 47 54 43 47 41 43 54 43 |GCCTGCAG|GTCGACTC|
|000000c0| 54 41 47 41 47 47 41 54 | 43 43 43 43 47 47 47 54 |TAGAGGAT|CCCCGGGT|
|000000d0| 41 43 43 47 41 47 43 54 | 43 47 41 41 54 54 43 43 |ACCGAGCT|CGAATTCC|
|000000e0| 47 47 54 43 54 43 43 43 | 54 41 54 41 47 54 47 41 |GGTCTCCC|TATAGTGA|
|000000f0| 47 54 43 47 54 41 54 54 | 41 41 54 54 54 43 47 41 |GTCGTATT|AATTTCGA|
|00000100| 54 41 41 47 43 43 41 47 | 43 54 47 43 41 54 54 41 |TAAGCCAG|CTGCATTA|
|00000110| 41 54 47 41 41 54 43 47 | 47 43 43 41 41 43 47 43 |ATGAATCG|GCCAACGC|
|00000120| 47 43 47 47 47 47 41 47 | 41 47 47 43 47 47 54 54 |GCGGGGAG|AGGCGGTT|
|00000130| 54 47 43 47 54 41 54 54 | 47 47 47 43 47 43 54 43 |TGCGTATT|GGGCGCTC|
|00000140| 54 54 43 43 47 43 54 54 | 43 43 54 43 47 43 54 43 |TTCCGCTT|CCTCGCTC|
|00000150| 41 43 54 47 41 43 54 43 | 47 43 54 47 43 47 43 54 |ACTGACTC|GCTGCGCT|
|00000160| 43 47 47 54 43 47 54 54 | 43 47 47 43 54 47 43 47 |CGGTCGTT|CGGCTGCG|
|00000170| 47 43 47 41 47 43 47 47 | 54 41 54 43 41 47 43 54 |GCGAGCGG|TATCAGCT|
|00000180| 43 41 43 54 43 41 41 41 | 47 47 43 47 47 54 41 41 |CACTCAAA|GGCGGTAA|
|00000190| 54 41 43 47 47 54 54 41 | 54 43 43 41 43 41 47 41 |TACGGTTA|TCCACAGA|
|000001a0| 41 54 43 41 47 47 47 47 | 41 54 41 41 43 47 43 41 |ATCAGGGG|ATAACGCA|
|000001b0| 47 47 41 41 41 47 41 41 | 43 41 54 47 54 47 41 47 |GGAAAGAA|CATGTGAG|
|000001c0| 43 41 41 41 41 47 47 43 | 43 41 47 43 41 41 41 41 |CAAAAGGC|CAGCAAAA|
|000001d0| 47 47 43 43 41 47 47 41 | 41 43 43 47 54 41 41 41 |GGCCAGGA|ACCGTAAA|
|000001e0| 41 41 47 47 43 43 47 43 | 47 54 54 47 43 54 47 47 |AAGGCCGC|GTTGCTGG|
|000001f0| 43 47 54 54 54 54 54 43 | 43 41 54 41 47 47 43 54 |CGTTTTTC|CATAGGCT|
|00000200| 43 43 47 43 43 43 43 43 | 43 54 47 41 43 47 41 47 |CCGCCCCC|CTGACGAG|
|00000210| 43 41 54 43 41 43 41 41 | 41 41 41 54 43 47 41 43 |CATCACAA|AAATCGAC|
|00000220| 47 43 54 43 41 41 47 54 | 43 41 47 41 47 47 54 47 |GCTCAAGT|CAGAGGTG|
|00000230| 47 43 47 41 41 41 43 43 | 43 47 41 43 41 47 47 41 |GCGAAACC|CGACAGGA|
|00000240| 43 54 41 54 41 41 41 47 | 41 54 41 43 43 41 47 47 |CTATAAAG|ATACCAGG|
|00000250| 43 47 54 54 54 43 43 43 | 43 43 54 47 47 41 41 47 |CGTTTCCC|CCTGGAAG|
|00000260| 43 54 43 43 43 54 43 47 | 54 47 43 47 43 54 43 54 |CTCCCTCG|TGCGCTCT|
|00000270| 43 43 54 47 54 54 43 43 | 47 41 43 43 43 54 47 43 |CCTGTTCC|GACCCTGC|
|00000280| 43 47 43 54 54 41 43 43 | 47 47 41 54 41 43 43 54 |CGCTTACC|GGATACCT|
|00000290| 47 54 43 43 47 43 43 54 | 54 54 43 54 43 43 43 54 |GTCCGCCT|TTCTCCCT|
|000002a0| 54 43 47 47 47 41 41 47 | 43 47 54 47 47 43 47 43 |TCGGGAAG|CGTGGCGC|
|000002b0| 54 54 54 43 54 43 41 54 | 41 47 43 54 43 41 43 47 |TTTCTCAT|AGCTCACG|
|000002c0| 43 54 47 54 41 47 47 54 | 41 54 43 54 43 41 47 54 |CTGTAGGT|ATCTCAGT|
|000002d0| 54 43 47 47 54 47 54 41 | 47 47 54 43 47 54 54 43 |TCGGTGTA|GGTCGTTC|
|000002e0| 47 43 54 43 43 41 41 47 | 43 54 47 47 47 43 54 47 |GCTCCAAG|CTGGGCTG|
|000002f0| 54 47 54 47 43 41 43 47 | 41 41 43 43 43 43 43 43 |TGTGCACG|AACCCCCC|
|00000300| 47 54 54 43 41 47 43 43 | 43 47 41 43 43 47 43 54 |GTTCAGCC|CGACCGCT|
|00000310| 47 43 47 43 43 54 54 41 | 54 43 43 47 47 54 41 41 |GCGCCTTA|TCCGGTAA|
|00000320| 43 54 41 54 43 47 54 43 | 54 54 47 41 47 54 43 43 |CTATCGTC|TTGAGTCC|
|00000330| 41 41 43 43 43 47 47 54 | 41 41 47 41 43 41 43 47 |AACCCGGT|AAGACACG|
|00000340| 41 43 54 54 41 54 43 47 | 43 43 41 43 54 47 47 43 |ACTTATCG|CCACTGGC|
|00000350| 41 47 43 41 47 43 43 41 | 43 54 47 47 54 41 41 43 |AGCAGCCA|CTGGTAAC|
|00000360| 41 47 47 41 54 54 41 47 | 43 41 47 41 47 43 47 41 |AGGATTAG|CAGAGCGA|
|00000370| 47 47 54 41 54 47 54 41 | 47 47 43 47 47 54 47 43 |GGTATGTA|GGCGGTGC|
|00000380| 54 41 43 41 47 41 47 54 | 54 43 54 54 47 41 41 47 |TACAGAGT|TCTTGAAG|
|00000390| 54 47 47 54 47 47 43 43 | 54 41 41 43 54 41 43 47 |TGGTGGCC|TAACTACG|
|000003a0| 47 43 54 41 43 41 43 54 | 41 47 41 41 47 47 41 43 |GCTACACT|AGAAGGAC|
|000003b0| 41 47 54 41 54 54 54 47 | 47 54 41 54 43 54 47 43 |AGTATTTG|GTATCTGC|
|000003c0| 47 43 54 43 54 47 43 54 | 47 41 41 47 43 43 41 47 |GCTCTGCT|GAAGCCAG|
|000003d0| 54 54 41 43 43 54 54 43 | 47 47 41 41 41 41 41 47 |TTACCTTC|GGAAAAAG|
|000003e0| 41 47 54 54 47 47 54 41 | 47 43 54 43 54 54 47 41 |AGTTGGTA|GCTCTTGA|
|000003f0| 54 43 43 47 47 43 41 41 | 41 43 41 41 41 43 43 41 |TCCGGCAA|ACAAACCA|
|00000400| 43 43 47 43 54 47 47 54 | 41 47 43 47 47 54 47 47 |CCGCTGGT|AGCGGTGG|
|00000410| 54 54 54 54 54 54 54 47 | 54 54 54 47 43 41 41 47 |TTTTTTTG|TTTGCAAG|
|00000420| 43 41 47 43 41 47 41 54 | 54 41 43 47 43 47 43 41 |CAGCAGAT|TACGCGCA|
|00000430| 47 41 41 41 41 41 41 41 | 47 47 41 54 43 54 43 41 |GAAAAAAA|GGATCTCA|
|00000440| 41 47 41 41 47 41 54 43 | 43 54 54 54 47 41 54 43 |AGAAGATC|CTTTGATC|
|00000450| 54 54 54 54 43 54 41 43 | 47 47 47 47 54 43 54 47 |TTTTCTAC|GGGGTCTG|
|00000460| 41 43 47 43 54 43 41 47 | 54 47 47 41 41 43 47 41 |ACGCTCAG|TGGAACGA|
|00000470| 41 41 41 43 54 43 41 43 | 47 54 54 41 41 47 47 47 |AAACTCAC|GTTAAGGG|
|00000480| 41 54 54 54 54 47 47 54 | 43 41 54 47 41 47 41 54 |ATTTTGGT|CATGAGAT|
|00000490| 54 41 54 43 41 41 41 41 | 41 47 47 41 54 43 54 54 |TATCAAAA|AGGATCTT|
|000004a0| 43 41 43 43 54 41 47 41 | 54 43 43 54 54 54 54 41 |CACCTAGA|TCCTTTTA|
|000004b0| 41 41 54 54 41 41 41 41 | 41 54 47 41 41 47 54 54 |AATTAAAA|ATGAAGTT|
|000004c0| 54 54 41 41 41 54 43 41 | 41 54 43 54 41 41 41 47 |TTAAATCA|ATCTAAAG|
|000004d0| 54 41 54 41 54 41 54 47 | 41 47 54 41 41 41 43 54 |TATATATG|AGTAAACT|
|000004e0| 54 47 47 54 43 54 47 41 | 43 41 47 54 54 41 43 43 |TGGTCTGA|CAGTTACC|
|000004f0| 41 41 54 47 43 54 54 41 | 41 54 43 41 47 54 47 41 |AATGCTTA|ATCAGTGA|
|00000500| 47 47 43 41 43 43 54 41 | 54 43 54 43 41 47 43 47 |GGCACCTA|TCTCAGCG|
|00000510| 41 54 43 54 47 54 43 54 | 41 54 54 54 43 47 54 54 |ATCTGTCT|ATTTCGTT|
|00000520| 43 41 54 43 43 41 54 41 | 47 54 54 47 43 43 54 47 |CATCCATA|GTTGCCTG|
|00000530| 41 43 54 43 43 43 43 47 | 54 43 47 54 47 54 41 47 |ACTCCCCG|TCGTGTAG|
|00000540| 41 54 41 41 43 54 41 43 | 47 41 54 41 43 47 47 47 |ATAACTAC|GATACGGG|
|00000550| 41 47 47 47 43 54 54 41 | 43 43 41 54 43 54 47 47 |AGGGCTTA|CCATCTGG|
|00000560| 43 43 43 43 41 47 54 47 | 43 54 47 43 41 41 54 47 |CCCCAGTG|CTGCAATG|
|00000570| 41 54 41 43 43 47 43 47 | 41 47 41 43 43 43 41 43 |ATACCGCG|AGACCCAC|
|00000580| 47 43 54 43 41 43 43 47 | 47 43 54 43 43 41 47 41 |GCTCACCG|GCTCCAGA|
|00000590| 54 54 54 41 54 43 41 47 | 43 41 41 54 41 41 41 43 |TTTATCAG|CAATAAAC|
|000005a0| 43 41 47 43 43 41 47 43 | 43 47 47 41 41 47 47 47 |CAGCCAGC|CGGAAGGG|
|000005b0| 43 43 47 41 47 43 47 43 | 41 47 41 41 47 54 47 47 |CCGAGCGC|AGAAGTGG|
|000005c0| 54 43 43 54 47 43 41 41 | 43 54 54 54 41 54 43 43 |TCCTGCAA|CTTTATCC|
|000005d0| 47 43 43 54 43 43 41 54 | 43 43 41 47 54 43 54 41 |GCCTCCAT|CCAGTCTA|
|000005e0| 54 54 41 41 54 54 47 54 | 54 47 43 43 47 47 47 41 |TTAATTGT|TGCCGGGA|
|000005f0| 41 47 43 54 41 47 41 47 | 54 41 41 47 54 41 47 54 |AGCTAGAG|TAAGTAGT|
|00000600| 54 43 47 43 43 41 47 54 | 54 41 41 54 41 47 54 54 |TCGCCAGT|TAATAGTT|
|00000610| 54 47 43 47 43 41 41 43 | 47 54 54 47 54 54 47 43 |TGCGCAAC|GTTGTTGC|
|00000620| 43 41 54 54 47 43 54 41 | 43 41 47 47 43 41 54 43 |CATTGCTA|CAGGCATC|
|00000630| 47 54 47 47 54 47 54 43 | 41 43 47 43 54 43 47 54 |GTGGTGTC|ACGCTCGT|
|00000640| 43 47 54 54 54 47 47 54 | 41 54 47 47 43 54 54 43 |CGTTTGGT|ATGGCTTC|
|00000650| 41 54 54 43 41 47 43 54 | 43 43 47 47 54 54 43 43 |ATTCAGCT|CCGGTTCC|
|00000660| 43 41 41 43 47 41 54 43 | 41 41 47 47 43 47 41 47 |CAACGATC|AAGGCGAG|
|00000670| 54 54 41 43 41 54 47 41 | 54 43 43 43 43 43 41 54 |TTACATGA|TCCCCCAT|
|00000680| 47 54 54 47 54 47 43 41 | 41 41 41 41 41 47 43 47 |GTTGTGCA|AAAAAGCG|
|00000690| 47 54 54 41 47 43 54 43 | 43 54 54 43 47 47 54 43 |GTTAGCTC|CTTCGGTC|
|000006a0| 43 54 43 43 47 41 54 43 | 47 54 54 47 54 43 41 47 |CTCCGATC|GTTGTCAG|
|000006b0| 41 41 47 54 41 41 47 54 | 54 47 47 43 43 47 43 41 |AAGTAAGT|TGGCCGCA|
|000006c0| 47 54 47 54 54 41 54 43 | 41 43 54 43 41 54 47 47 |GTGTTATC|ACTCATGG|
|000006d0| 54 54 41 54 47 47 43 41 | 47 43 41 43 54 47 43 41 |TTATGGCA|GCACTGCA|
|000006e0| 54 41 41 54 54 43 54 43 | 54 54 41 43 54 47 54 43 |TAATTCTC|TTACTGTC|
|000006f0| 41 54 47 43 43 41 54 43 | 43 47 54 41 41 47 41 54 |ATGCCATC|CGTAAGAT|
|00000700| 47 43 54 54 54 54 43 54 | 47 54 47 41 43 54 47 47 |GCTTTTCT|GTGACTGG|
|00000710| 54 47 41 47 54 41 43 54 | 43 41 41 43 43 41 41 47 |TGAGTACT|CAACCAAG|
|00000720| 54 43 41 54 54 43 54 47 | 41 47 41 41 54 41 47 54 |TCATTCTG|AGAATAGT|
|00000730| 47 54 41 54 47 43 47 47 | 43 47 41 43 43 47 41 47 |GTATGCGG|CGACCGAG|
|00000740| 54 54 47 43 54 43 54 54 | 47 43 43 43 47 47 43 47 |TTGCTCTT|GCCCGGCG|
|00000750| 54 43 41 41 54 41 43 47 | 47 47 41 54 41 41 54 41 |TCAATACG|GGATAATA|
|00000760| 43 43 47 43 47 43 43 41 | 43 41 54 41 47 43 41 47 |CCGCGCCA|CATAGCAG|
|00000770| 41 41 43 54 54 54 41 41 | 41 41 47 54 47 43 54 43 |AACTTTAA|AAGTGCTC|
|00000780| 41 54 43 41 54 54 47 47 | 41 41 41 41 43 47 54 54 |ATCATTGG|AAAACGTT|
|00000790| 43 54 54 43 47 47 47 47 | 43 47 41 41 41 41 43 54 |CTTCGGGG|CGAAAACT|
|000007a0| 43 54 43 41 41 47 47 41 | 54 43 54 54 41 43 43 47 |CTCAAGGA|TCTTACCG|
|000007b0| 43 54 47 54 54 47 41 47 | 41 54 43 43 41 47 54 54 |CTGTTGAG|ATCCAGTT|
|000007c0| 43 47 41 54 47 54 41 41 | 43 43 43 41 43 54 43 47 |CGATGTAA|CCCACTCG|
|000007d0| 54 47 43 41 43 43 43 41 | 41 43 54 47 41 54 43 54 |TGCACCCA|ACTGATCT|
|000007e0| 54 43 41 47 43 41 54 43 | 54 54 54 54 41 43 54 54 |TCAGCATC|TTTTACTT|
|000007f0| 54 43 41 43 43 41 47 43 | 47 54 54 54 43 54 47 47 |TCACCAGC|GTTTCTGG|
|00000800| 47 54 47 41 47 43 41 41 | 41 41 41 43 41 47 47 41 |GTGAGCAA|AAACAGGA|
|00000810| 41 47 47 43 41 41 41 41 | 54 47 43 43 47 43 41 41 |AGGCAAAA|TGCCGCAA|
|00000820| 41 41 41 41 47 47 47 41 | 41 54 41 41 47 47 47 43 |AAAAGGGA|ATAAGGGC|
|00000830| 47 41 43 41 43 47 47 41 | 41 41 54 47 54 54 47 41 |GACACGGA|AATGTTGA|
|00000840| 41 54 41 43 54 43 41 54 | 41 43 54 43 54 54 43 43 |ATACTCAT|ACTCTTCC|
|00000850| 54 54 54 54 54 43 41 41 | 54 41 54 54 41 54 54 47 |TTTTTCAA|TATTATTG|
|00000860| 41 41 47 43 41 54 54 54 | 41 54 43 41 47 47 47 54 |AAGCATTT|ATCAGGGT|
|00000870| 54 41 54 54 47 54 43 54 | 43 41 54 47 41 47 43 47 |TATTGTCT|CATGAGCG|
|00000880| 47 41 54 41 43 41 47 54 | 54 47 41 47 41 54 43 43 |GATACAGT|TGAGATCC|
|00000890| 41 47 54 54 43 47 41 54 | 47 54 41 41 43 43 43 41 |AGTTCGAT|GTAACCCA|
|000008a0| 43 54 43 47 54 47 43 41 | 43 43 43 41 41 43 54 47 |CTCGTGCA|CCCAACTG|
|000008b0| 41 54 43 54 54 43 41 47 | 43 41 54 43 54 54 54 54 |ATCTTCAG|CATCTTTT|
|000008c0| 41 43 54 54 54 43 41 43 | 43 41 47 43 47 54 54 54 |ACTTTCAC|CAGCGTTT|
|000008d0| 43 54 47 47 47 54 47 41 | 47 43 41 41 41 41 41 43 |CTGGGTGA|GCAAAAAC|
|000008e0| 41 47 47 41 41 47 47 43 | 41 41 41 41 54 47 43 43 |AGGAAGGC|AAAATGCC|
|000008f0| 47 43 41 41 41 41 41 41 | 47 47 47 41 41 54 41 41 |GCAAAAAA|GGGAATAA|
|00000900| 47 47 47 43 47 41 43 41 | 43 47 47 41 41 41 54 47 |GGGCGACA|CGGAAATG|
|00000910| 54 54 47 41 41 54 41 43 | 54 43 41 54 41 43 54 43 |TTGAATAC|TCATACTC|
|00000920| 54 54 43 43 54 54 54 54 | 54 43 41 41 54 41 54 54 |TTCCTTTT|TCAATATT|
|00000930| 41 54 54 47 41 41 47 43 | 41 54 54 54 41 54 43 41 |ATTGAAGC|ATTTATCA|
|00000940| 47 47 47 54 54 41 54 54 | 47 54 43 54 43 41 54 47 |GGGTTATT|GTCTCATG|
|00000950| 41 47 43 47 47 41 54 41 | 43 41 54 41 54 54 54 47 |AGCGGATA|CATATTTG|
|00000960| 41 41 54 47 54 41 54 54 | 54 41 47 41 41 41 41 41 |AATGTATT|TAGAAAAA|
|00000970| 54 41 41 41 43 41 41 41 | 54 41 47 47 47 47 54 54 |TAAACAAA|TAGGGGTT|
|00000980| 43 43 47 43 47 43 41 43 | 41 54 54 54 43 43 43 43 |CCGCGCAC|ATTTCCCC|
|00000990| 47 41 41 41 41 47 54 47 | 43 43 41 43 43 54 47 41 |GAAAAGTG|CCACCTGA|
|000009a0| 43 47 54 43 54 41 41 47 | 41 41 41 43 43 41 54 54 |CGTCTAAG|AAACCATT|
|000009b0| 41 54 54 41 54 43 41 54 | 47 41 43 41 54 54 41 41 |ATTATCAT|GACATTAA|
|000009c0| 43 43 54 41 54 41 41 41 | 41 41 54 41 47 47 43 47 |CCTATAAA|AATAGGCG|
|000009d0| 54 41 54 43 41 43 47 41 | 47 47 43 43 43 54 54 54 |TATCACGA|GGCCCTTT|
|000009e0| 43 47 54 43 54 43 47 43 | 47 43 47 54 54 54 43 47 |CGTCTCGC|GCGTTTCG|
|000009f0| 47 54 47 41 54 47 41 43 | 47 47 54 47 41 41 54 47 |GTGATGAC|GGTGAATG|
|00000a00| 41 47 47 43 43 47 54 54 | 47 41 47 43 41 43 43 47 |AGGCCGTT|GAGCACCG|
|00000a10| 43 43 47 43 43 47 43 41 | 41 47 47 41 41 54 47 47 |CCGCCGCA|AGGAATGG|
|00000a20| 54 47 43 41 41 47 47 41 | 47 41 54 47 47 43 47 43 |TGCAAGGA|GATGGCGC|
|00000a30| 43 43 41 41 43 41 47 54 | 43 43 43 43 43 47 47 43 |CCAACAGT|CCCCCGGC|
|00000a40| 43 41 43 47 47 47 47 43 | 43 54 47 43 43 41 43 43 |CACGGGGC|CTGCCACC|
|00000a50| 41 54 41 43 43 43 41 43 | 47 43 43 47 41 41 41 43 |ATACCCAC|GCCGAAAC|
|00000a60| 41 41 47 43 47 43 54 43 | 41 54 47 41 47 43 43 43 |AAGCGCTC|ATGAGCCC|
|00000a70| 47 41 41 47 54 47 47 43 | 47 41 47 43 43 43 47 41 |GAAGTGGC|GAGCCCGA|
|00000a80| 54 43 54 54 43 43 43 43 | 41 54 43 47 47 54 47 41 |TCTTCCCC|ATCGGTGA|
|00000a90| 54 47 54 43 47 47 43 47 | 41 54 41 54 41 47 47 43 |TGTCGGCG|ATATAGGC|
|00000aa0| 47 43 43 41 47 43 41 41 | 43 43 47 43 41 43 43 54 |GCCAGCAA|CCGCACCT|
|00000ab0| 47 54 47 47 43 47 43 43 | 47 47 54 47 41 54 47 43 |GTGGCGCC|GGTGATGC|
|00000ac0| 43 47 47 43 43 41 43 47 | 41 54 47 43 47 54 43 43 |CGGCCACG|ATGCGTCC|
|00000ad0| 47 47 43 47 54 41 47 41 | 47 47 41 54 43 54 47 47 |GGCGTAGA|GGATCTGG|
|00000ae0| 43 54 41 47 43 47 41 54 | 47 41 43 43 43 54 47 43 |CTAGCGAT|GACCCTGC|
|00000af0| 54 47 41 54 54 47 47 54 | 54 43 47 43 54 47 41 43 |TGATTGGT|TCGCTGAC|
|00000b00| 43 41 54 54 54 43 43 47 | 47 47 47 54 47 43 47 47 |CATTTCCG|GGGTGCGG|
|00000b10| 41 41 43 47 47 43 47 54 | 54 41 43 43 41 47 41 41 |AACGGCGT|TACCAGAA|
|00000b20| 41 43 54 43 41 47 41 41 | 47 47 54 54 43 47 54 43 |ACTCAGAA|GGTTCGTC|
|00000b30| 43 41 41 43 43 41 41 41 | 43 43 47 41 43 54 43 54 |CAACCAAA|CCGACTCT|
|00000b40| 47 41 43 47 47 43 41 47 | 54 54 54 41 43 47 41 47 |GACGGCAG|TTTACGAG|
|00000b50| 41 47 41 47 41 54 47 41 | 54 41 47 47 47 54 43 54 |AGAGATGA|TAGGGTCT|
|00000b60| 47 43 54 54 43 41 47 54 | 41 41 47 43 43 41 47 41 |GCTTCAGT|AAGCCAGA|
|00000b70| 54 47 43 54 41 43 41 43 | 41 41 54 54 41 47 47 43 |TGCTACAC|AATTAGGC|
|00000b80| 54 54 47 54 41 43 41 54 | 41 54 54 47 54 43 47 54 |TTGTACAT|ATTGTCGT|
|00000b90| 54 41 47 41 41 43 47 43 | 47 47 43 54 41 43 41 41 |TAGAACGC|GGCTACAA|
|00000ba0| 54 54 41 41 54 41 43 41 | 54 41 41 43 43 54 54 41 |TTAATACA|TAACCTTA|
|00000bb0| 54 47 54 41 54 43 41 54 | 41 43 41 43 41 54 41 43 |TGTATCAT|ACACATAC|
|00000bc0| 47 41 54 54 54 41 47 47 | 54 47 41 43 41 43 54 41 |GATTTAGG|TGACACTA|
|00000bd0| 54 41 00 00 01 0e 00 00 | 0b 32 00 06 00 00 00 00 |TA......|.2......|
|00000be0| 00 00 00 06 00 ba 00 04 | 00 a4 00 09 dd 6b 08 c2 |........|.....k..|
|00000bf0| 06 a2 01 70 ff ff ff ff | ff ff ff ff 00 07 00 00 |...p....|........|
|00000c00| 00 10 00 10 00 01 00 00 | 00 00 00 06 00 00 00 3f |........|.......?|
|00000c10| 00 ba 00 04 00 a4 00 09 | 00 00 00 00 d4 00 01 d4 |........|........|
|00000c20| ff ff 00 00 ff ff 00 00 | 00 09 00 00 00 10 00 10 |........|........|
|00000c30| 00 01 00 00 00 00 00 3f | 00 00 00 45 00 ba 00 04 |.......?|...E....|
|00000c40| 00 a4 00 09 dd 6b 08 c2 | 06 a2 01 70 ff ff ff ff |.....k..|...p....|
|00000c50| ff ff ff ff 00 07 00 00 | 00 10 00 10 00 01 00 00 |........|........|
|00000c60| 00 00 00 45 00 00 00 59 | 00 ba 00 04 00 a4 00 09 |...E...Y|........|
|00000c70| 00 00 80 00 11 b0 01 44 | aa 55 aa 55 aa 55 aa 55 |.......D|.U.U.U.U|
|00000c80| 00 07 00 00 00 10 00 10 | 00 01 00 00 00 00 00 59 |........|.......Y|
|00000c90| 00 00 0b 20 00 ba 00 04 | 00 a4 00 09 dd 6b 08 c2 |... ....|.....k..|
|00000ca0| 06 a2 01 70 ff ff ff ff | ff ff ff ff 00 07 00 00 |...p....|........|
|00000cb0| 00 10 00 10 00 01 00 00 | 00 00 0b 20 00 00 0b 32 |........|... ...2|
|00000cc0| 00 ba 00 04 00 a4 00 09 | 00 00 80 00 11 b0 01 48 |........|.......H|
|00000cd0| aa 55 aa 55 aa 55 aa 55 | 00 07 00 00 00 10 00 10 |.U.U.U.U|........|
|00000ce0| 00 01 00 00 00 00 00 ce | 00 00 0b 32 00 04 00 00 |........|...2....|
|00000cf0| 00 06 00 00 00 3f 00 28 | 00 00 00 0c 00 00 00 00 |.....?.(|........|
|00000d00| 00 01 00 42 00 08 00 06 | 00 04 00 32 00 09 00 00 |...B....|...2....|
|00000d10| 00 00 00 00 00 58 15 b8 | 00 00 00 00 00 00 00 00 |.....X..|........|
|00000d20| 00 00 00 45 00 00 00 59 | 00 e7 00 00 00 0c 00 00 |...E...Y|........|
|00000d30| 00 00 00 01 00 59 00 08 | 00 06 00 04 00 42 00 09 |.....Y..|.....B..|
|00000d40| 00 00 00 00 00 00 00 58 | 15 74 00 58 14 0c 01 00 |.......X|.t.X....|
|00000d50| 00 00 00 00 04 4e 00 00 | 07 63 00 28 00 00 00 0c |.....N..|.c.(....|
|00000d60| 00 58 16 b0 00 01 01 42 | 00 0a 00 0d 00 04 00 32 |.X.....B|.......2|
|00000d70| 00 09 02 41 ab 54 ea ff | 00 58 15 e4 00 58 18 68 |...A.T..|.X...X.h|
|00000d80| 00 00 00 00 00 00 0b 20 | 00 00 0b 32 00 e7 00 00 |....... |...2....|
|00000d90| 00 0c 00 00 00 00 00 01 | 00 32 00 08 00 06 00 04 |........|.2......|
|00000da0| 00 42 00 09 00 00 00 00 | 00 00 00 58 16 18 00 58 |.B......|...X...X|
|00000db0| 15 d8 01 00 00 00 12 70 | 6f 6c 79 63 6c 6f 6e 69 |.......p|olycloni|
|00000dc0| 6e 67 20 72 65 67 69 6f | 6e 0b 54 37 20 50 72 6f |ng regio|n.T7 Pro|
|00000dd0| 6d 6f 74 65 72 00 00 00 | 1f 69 6e 20 63 6f 75 6e |moter...|.in coun|
|00000de0| 74 65 72 2d 63 6c 6f 63 | 6b 77 69 73 65 20 64 69 |ter-cloc|kwise di|
|00000df0| 72 65 63 74 69 6f 6e 2e | 15 41 6d 70 69 63 69 6c |rection.|.Ampicil|
|00000e00| 6c 69 6e 20 52 65 73 69 | 73 74 61 6e 63 65 00 00 |lin Resi|stance..|
|00000e10| 00 10 a7 2d 6c 61 63 74 | 61 6d 61 73 65 20 67 65 |...-lact|amase ge|
|00000e20| 6e 65 0c 53 50 36 20 50 | 72 6f 6d 6f 74 65 72 00 |ne.SP6 P|romoter.|
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|00001390| 67 0a 20 54 70 01 21 40 | 00 06 60 0a 20 54 21 7c |g. Tp.!@|..`. T!||
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|000013c0| 20 54 10 28 00 2b 48 80 | 05 70 47 45 4d 33 00 00 | T.(.+H.|.pGEM3..|
|000013d0| 09 49 54 68 69 73 20 66 | 69 6c 65 20 77 61 73 20 |.IThis f|ile was |
|000013e0| 64 6f 77 6e 6c 6f 61 64 | 65 64 20 66 72 6f 6d 20 |download|ed from |
|000013f0| 74 68 65 20 31 32 2f 38 | 37 20 56 65 63 42 61 73 |the 12/8|7 VecBas|
|00001400| 65 20 62 79 20 4b 65 69 | 74 68 20 41 20 4a 6f 68 |e by Kei|th A Joh|
|00001410| 6e 73 6f 6e 20 6f 6e 20 | 44 65 63 2e 20 31 34 2c |nson on |Dec. 14,|
|00001420| 20 31 39 38 39 2e 0d 4d | 6f 64 69 66 69 65 64 20 | 1989..M|odified |
|00001430| 62 79 20 42 6f 62 20 47 | 72 6f 73 73 2c 20 31 32 |by Bob G|ross, 12|
|00001440| 2f 32 36 2f 38 39 2e 0d | 0d 45 4e 54 52 59 20 50 |/26/89..|.ENTRY P|
|00001450| 47 45 4d 33 20 20 20 20 | 20 20 20 20 20 20 20 20 |GEM3    |        |
|00001460| 20 20 20 20 20 20 20 20 | 23 54 59 50 45 20 44 4e |        |#TYPE DN|
|00001470| 41 20 43 49 52 43 55 4c | 41 52 0d 54 49 54 4c 45 |A CIRCUL|AR.TITLE|
|00001480| 20 70 47 45 4d 33 20 2d | 20 43 6c 6f 6e 69 6e 67 | pGEM3 -| Cloning|
|00001490| 20 56 65 63 74 6f 72 20 | 47 65 6d 69 6e 69 20 33 | Vector |Gemini 3|
|000014a0| 20 66 72 6f 6d 20 50 72 | 6f 6d 65 67 61 20 42 69 | from Pr|omega Bi|
|000014b0| 6f 74 65 63 0d 44 41 54 | 45 20 20 20 20 20 20 32 |otec.DAT|E      2|
|000014c0| 37 2d 4d 41 52 2d 31 39 | 38 36 0d 20 20 20 23 73 |7-MAR-19|86.   #s|
|000014d0| 65 71 75 65 6e 63 65 20 | 20 31 31 2d 44 45 43 2d |equence | 11-DEC-|
|000014e0| 31 39 38 36 0d 20 20 20 | 23 73 65 71 75 65 6e 63 |1986.   |#sequenc|
|000014f0| 65 20 20 31 36 2d 44 45 | 43 2d 31 39 38 36 0d 41 |e  16-DE|C-1986.A|
|00001500| 43 43 45 53 53 49 4f 4e | 20 56 42 30 30 33 38 0d |CCESSION| VB0038.|
|00001510| 53 4f 55 52 43 45 20 20 | 20 20 61 72 74 69 66 69 |SOURCE  |  artifi|
|00001520| 63 69 61 6c 0d 52 45 46 | 45 52 45 4e 43 45 0d 20 |cial.REF|ERENCE. |
|00001530| 20 20 23 6e 75 6d 62 65 | 72 20 31 0d 20 20 20 23 |  #numbe|r 1.   #|
|00001540| 63 69 74 61 74 69 6f 6e | 20 20 50 72 6f 6d 65 67 |citation|  Promeg|
|00001550| 61 20 74 65 63 68 6e 69 | 63 61 6c 20 42 75 6c 6c |a techni|cal Bull|
|00001560| 65 74 69 6e 0d 20 20 20 | 23 63 6f 6d 6d 65 6e 74 |etin.   |#comment|
|00001570| 20 20 20 54 68 65 20 73 | 65 71 75 65 6e 63 65 20 |   The s|equence |
|00001580| 77 61 73 20 72 65 76 69 | 73 65 64 20 62 79 20 50 |was revi|sed by P|
|00001590| 72 6f 6d 65 67 61 20 42 | 69 6f 74 65 63 20 4a 55 |romega B|iotec JU|
|000015a0| 4c 2d 31 39 38 36 0d 20 | 20 20 20 20 20 20 20 20 |L-1986. |        |
|000015b0| 20 20 20 20 20 50 72 6f | 6d 65 67 61 20 61 6c 73 |     Pro|mega als|
|000015c0| 6f 20 63 6f 6e 66 69 72 | 6d 65 64 20 74 68 65 20 |o confir|med the |
|000015d0| 72 65 76 69 73 69 6f 6e | 20 6f 66 20 44 45 43 2d |revision| of DEC-|
|000015e0| 31 39 38 36 0d 20 20 20 | 23 63 6f 6d 6d 65 6e 74 |1986.   |#comment|
|000015f0| 20 20 63 6f 70 79 72 69 | 67 68 74 20 6f 66 20 74 |  copyri|ght of t|
|00001600| 68 65 20 73 65 71 75 65 | 6e 63 65 20 62 79 20 50 |he seque|nce by P|
|00001610| 72 6f 6d 65 67 61 0d 43 | 4f 4d 4d 45 4e 54 0d 20 |romega.C|OMMENT. |
|00001620| 20 20 45 6e 74 65 72 65 | 64 20 62 79 20 57 69 6c |  Entere|d by Wil|
|00001630| 6c 69 61 6d 20 47 69 6c | 62 65 72 74 2c 20 57 68 |liam Gil|bert, Wh|
|00001640| 69 74 61 6b 65 72 20 43 | 6f 6c 6c 65 67 65 2c 20 |itaker C|ollege, |
|00001650| 4d 49 54 0d 20 20 20 20 | 20 20 20 61 6e 64 20 62 |MIT.    |   and b|
|00001660| 79 20 46 2e 20 50 66 65 | 69 66 66 65 72 2c 20 4d |y F. Pfe|iffer, M|
|00001670| 50 49 2c 20 4d 61 72 74 | 69 6e 73 72 69 65 64 0d |PI, Mart|insried.|
|00001680| 20 20 20 52 65 76 69 73 | 65 64 20 31 31 2d 44 45 |   Revis|ed 11-DE|
|00001690| 43 2d 31 39 38 36 20 62 | 79 20 46 2e 20 50 66 65 |C-1986 b|y F. Pfe|
|000016a0| 69 66 66 65 72 20 61 63 | 63 6f 72 64 69 6e 67 20 |iffer ac|cording |
|000016b0| 74 6f 20 50 72 6f 6d 65 | 67 61 0d 20 20 20 52 65 |to Prome|ga.   Re|
|000016c0| 76 69 73 65 64 20 31 36 | 2d 44 45 43 2d 31 39 38 |vised 16|-DEC-198|
|000016d0| 36 20 62 79 20 46 2e 20 | 50 66 65 69 66 66 65 72 |6 by F. |Pfeiffer|
|000016e0| 3a 0d 20 20 20 35 33 36 | 2f 37 20 22 41 54 22 20 |:.   536|/7 "AT" |
|000016f0| 74 6f 20 22 54 41 22 20 | 74 6f 20 6d 61 74 63 68 |to "TA" |to match|
|00001700| 20 72 65 76 69 73 65 64 | 20 73 65 71 75 65 6e 63 | revised| sequenc|
|00001710| 65 20 6f 66 20 50 42 52 | 33 32 32 0d 43 4f 4d 4d |e of PBR|322.COMM|
|00001720| 45 4e 54 0d 20 20 20 54 | 68 65 20 70 47 45 4d 33 |ENT.   T|he pGEM3|
|00001730| 20 76 65 63 74 6f 72 20 | 63 6f 6e 74 61 69 6e 73 | vector |contains|
|00001740| 20 74 68 65 20 53 50 36 | 20 61 6e 64 20 54 37 20 | the SP6| and T7 |
|00001750| 70 72 6f 6d 6f 74 65 72 | 73 20 66 6c 61 6e 6b 69 |promoter|s flanki|
|00001760| 6e 67 0d 20 20 20 61 20 | 70 55 43 31 38 20 64 65 |ng.   a |pUC18 de|
|00001770| 72 69 76 65 64 20 6d 75 | 6c 74 69 70 6c 65 20 63 |rived mu|ltiple c|
|00001780| 6c 6f 6e 69 6e 67 20 73 | 69 74 65 2e 0d 4b 45 59 |loning s|ite..KEY|
|00001790| 57 4f 52 44 53 0d 43 52 | 4f 53 53 52 45 46 45 52 |WORDS.CR|OSSREFER|
|000017a0| 45 4e 43 45 0d 20 20 20 | 23 70 61 72 65 6e 74 0d |ENCE.   |#parent.|
|000017b0| 20 20 20 20 20 56 65 63 | 42 61 73 65 28 33 29 3a |     Vec|Base(3):|
|000017c0| 70 47 45 4d 31 0d 20 20 | 20 23 70 61 72 65 6e 74 |pGEM1.  | #parent|
|000017d0| 0d 20 20 20 20 20 56 65 | 63 42 61 73 65 28 33 29 |.     Ve|cBase(3)|
|000017e0| 3a 70 55 43 31 38 2c 20 | 56 65 63 53 6f 75 72 63 |:pUC18, |VecSourc|
|000017f0| 65 28 33 29 3a 62 47 61 | 6c 31 38 2c 0d 20 20 20 |e(3):bGa|l18,.   |
|00001800| 20 20 56 65 63 53 6f 75 | 72 63 65 28 33 29 3a 50 |  VecSou|rce(3):P|
|00001810| 72 6f 6d 53 50 36 2c 20 | 56 65 63 53 6f 75 72 63 |romSP6, |VecSourc|
|00001820| 65 28 33 29 3a 50 72 6f | 6d 54 37 2c 0d 20 20 20 |e(3):Pro|mT7,.   |
|00001830| 20 20 56 65 63 42 61 73 | 65 28 33 29 3a 70 42 52 |  VecBas|e(3):pBR|
|00001840| 33 32 32 0d 20 20 20 23 | 62 72 6f 74 68 65 72 0d |322.   #|brother.|
|00001850| 20 20 20 20 20 56 65 63 | 42 61 73 65 28 33 29 3a |     Vec|Base(3):|
|00001860| 70 47 45 4d 34 0d 50 41 | 52 45 4e 54 0d 20 20 20 |pGEM4.PA|RENT.   |
|00001870| 46 65 61 74 75 72 65 73 | 20 6f 66 20 70 47 45 4d |Features| of pGEM|
|00001880| 33 20 28 32 38 36 37 20 | 62 70 29 0d 20 20 20 20 |3 (2867 |bp).    |
|00001890| 20 72 65 73 69 64 75 65 | 20 20 20 20 20 73 6f 75 | residue|     sou|
|000018a0| 72 63 65 0d 20 20 20 20 | 20 20 37 2d 20 20 36 33 |rce.    |  7-  63|
|000018b0| 20 20 20 20 35 37 2d 20 | 20 20 31 20 28 63 29 20 |    57- |  1 (c) |
|000018c0| 4d 31 33 6d 70 31 38 2f | 70 55 43 31 38 2d 70 6f |M13mp18/|pUC18-po|
|000018d0| 6c 79 6c 69 6e 6b 65 72 | 0d 20 20 20 20 20 37 30 |lylinker|.     70|
|000018e0| 2d 20 20 38 39 20 20 20 | 20 32 30 2d 20 20 20 31 |-  89   | 20-   1|
|000018f0| 20 28 63 29 20 54 37 20 | 70 72 6f 6d 6f 74 65 72 | (c) T7 |promoter|
|00001900| 0d 20 20 20 20 31 30 30 | 2d 20 31 35 38 20 20 20 |.    100|- 158   |
|00001910| 20 35 39 2d 20 20 20 31 | 20 28 63 29 20 70 55 43 | 59-   1| (c) pUC|
|00001920| 31 38 0d 20 20 20 20 31 | 35 39 2d 32 33 34 37 20 |18.    1|59-2347 |
|00001930| 20 32 36 38 36 2d 20 34 | 39 38 20 28 63 29 20 70 | 2686- 4|98 (c) p|
|00001940| 55 43 31 38 0d 20 20 20 | 32 33 34 38 2d 32 34 33 |UC18.   |2348-243|
|00001950| 33 20 20 20 36 35 34 2d | 20 35 36 39 20 28 63 29 |3   654-| 569 (c)|
|00001960| 20 70 42 52 33 32 32 0d | 20 20 20 32 34 33 34 2d | pBR322.|   2434-|
|00001970| 32 36 32 32 20 20 20 35 | 36 34 2d 20 33 37 36 20 |2622   5|64- 376 |
|00001980| 28 63 29 20 70 42 52 33 | 32 32 0d 20 20 20 32 36 |(c) pBR3|22.   26|
|00001990| 32 33 2d 32 38 36 37 20 | 20 78 78 78 78 2d 78 78 |23-2867 | xxxx-xx|
|000019a0| 78 78 20 20 20 20 20 70 | 68 61 67 65 20 53 50 36 |xx     p|hage SP6|
|000019b0| 0d 20 20 20 32 38 34 39 | 2d 32 38 36 37 20 20 20 |.   2849|-2867   |
|000019c0| 20 20 31 2d 20 20 31 39 | 20 20 20 20 20 53 50 36 |  1-  19|     SP6|
|000019d0| 20 70 72 6f 6d 6f 74 65 | 72 0d 20 20 20 43 6f 6e | promote|r.   Con|
|000019e0| 66 6c 69 63 74 20 28 63 | 66 6c 29 20 61 6e 64 20 |flict (c|fl) and |
|000019f0| 4d 75 74 61 74 69 6f 6e | 73 20 28 6d 75 74 29 3a |Mutation|s (mut):|
|00001a00| 20 6e 6f 6e 65 0d 50 41 | 52 45 4e 54 0d 20 20 20 | none.PA|RENT.   |
|00001a10| 46 65 61 74 75 72 65 73 | 20 6f 66 20 70 47 45 4d |Features| of pGEM|
|00001a20| 33 20 28 32 38 36 37 20 | 62 70 29 0d 20 20 20 20 |3 (2867 |bp).    |
|00001a30| 20 72 65 73 69 64 75 65 | 20 20 20 20 20 73 6f 75 | residue|     sou|
|00001a40| 72 63 65 0d 20 20 20 20 | 20 20 37 2d 20 20 36 33 |rce.    |  7-  63|
|00001a50| 20 20 20 20 35 37 2d 20 | 20 20 31 20 28 63 29 20 |    57- |  1 (c) |
|00001a60| 4d 31 33 6d 70 31 38 2f | 70 55 43 31 38 2d 70 6f |M13mp18/|pUC18-po|
|00001a70| 6c 79 6c 69 6e 6b 65 72 | 0d 20 20 20 20 20 37 30 |lylinker|.     70|
|00001a80| 2d 20 20 38 39 20 20 20 | 20 32 30 2d 20 20 20 31 |-  89   | 20-   1|
|00001a90| 20 28 63 29 20 54 37 20 | 70 72 6f 6d 6f 74 65 72 | (c) T7 |promoter|
|00001aa0| 0d 20 20 20 20 31 30 30 | 2d 32 31 36 30 20 20 20 |.    100|-2160   |
|00001ab0| 36 32 36 2d 32 36 38 36 | 20 20 20 20 20 70 55 43 |626-2686|     pUC|
|00001ac0| 31 38 63 0d 20 20 20 32 | 31 36 31 2d 32 33 34 37 |18c.   2|161-2347|
|00001ad0| 20 20 20 20 20 31 2d 20 | 31 38 37 20 20 20 20 20 |     1- |187     |
|00001ae0| 70 55 43 31 38 63 0d 20 | 20 20 32 33 34 38 2d 32 |pUC18c. |  2348-2|
|00001af0| 34 33 33 20 20 20 36 35 | 34 2d 20 35 36 39 20 28 |433   65|4- 569 (|
|00001b00| 63 29 20 70 42 52 33 32 | 32 0d 20 20 20 32 34 33 |c) pBR32|2.   243|
|00001b10| 34 2d 32 36 32 32 20 20 | 20 35 36 34 2d 20 33 37 |4-2622  | 564- 37|
|00001b20| 36 20 28 63 29 20 70 42 | 52 33 32 32 0d 20 20 20 |6 (c) pB|R322.   |
|00001b30| 32 36 32 33 2d 32 38 36 | 37 20 20 78 78 78 78 2d |2623-286|7  xxxx-|
|00001b40| 78 78 78 78 20 20 20 20 | 20 70 68 61 67 65 20 53 |xxxx    | phage S|
|00001b50| 50 36 0d 20 20 20 32 38 | 34 39 2d 32 38 36 37 20 |P6.   28|49-2867 |
|00001b60| 20 20 20 20 31 2d 20 20 | 31 39 20 20 20 20 20 53 |    1-  |19     S|
|00001b70| 50 36 20 70 72 6f 6d 6f | 74 65 72 0d 20 20 20 43 |P6 promo|ter.   C|
|00001b80| 6f 6e 66 6c 69 63 74 20 | 28 63 66 6c 29 20 61 6e |onflict |(cfl) an|
|00001b90| 64 20 4d 75 74 61 74 69 | 6f 6e 73 20 28 6d 75 74 |d Mutati|ons (mut|
|00001ba0| 29 3a 20 6e 6f 6e 65 0d | 46 45 41 54 55 52 45 0d |): none.|FEATURE.|
|00001bb0| 20 20 20 20 20 20 20 20 | 20 20 20 31 20 20 20 20 |        |   1    |
|00001bc0| 20 20 20 20 20 20 20 73 | 74 61 72 74 20 6f 66 20 |       s|tart of |
|00001bd0| 53 50 36 2d 52 4e 41 20 | 73 79 6e 74 68 65 73 69 |SP6-RNA |synthesi|
|00001be0| 73 0d 20 20 20 20 20 20 | 20 20 20 20 37 32 20 20 |s.      |    72  |
|00001bf0| 20 20 20 20 20 28 63 29 | 20 73 74 61 72 74 20 6f |     (c)| start o|
|00001c00| 66 20 54 37 2d 52 4e 41 | 20 73 79 6e 74 68 65 73 |f T7-RNA| synthes|
|00001c10| 69 73 0d 20 20 20 31 31 | 30 33 2d 31 38 39 31 20 |is.   11|03-1891 |
|00001c20| 37 38 39 2d 31 20 28 63 | 29 20 41 70 2d 52 3b 20 |789-1 (c|) Ap-R; |
|00001c30| 62 2d 6c 61 63 74 61 6d | 61 73 65 0d 50 4f 4c 59 |b-lactam|ase.POLY|
|00001c40| 4c 49 4e 4b 45 52 20 20 | 45 63 6f 52 49 2d 53 61 |LINKER  |EcoRI-Sa|
|00001c50| 63 49 2d 4b 70 6e 49 2d | 53 6d 61 49 2d 42 61 6d |cI-KpnI-|SmaI-Bam|
|00001c60| 48 49 2d 58 62 61 49 2d | 53 61 6c 49 2d 50 73 74 |HI-XbaI-|SalI-Pst|
|00001c70| 49 2d 53 70 68 49 2d 48 | 69 6e 64 49 49 49 0d 53 |I-SphI-H|indIII.S|
|00001c80| 45 4c 45 43 54 49 4f 4e | 20 23 72 65 73 69 73 74 |ELECTION| #resist|
|00001c90| 61 6e 63 65 20 20 41 70 | 0d 53 55 4d 4d 41 52 59 |ance  Ap|.SUMMARY|
|00001ca0| 20 20 70 47 45 4d 33 20 | 20 20 20 23 6c 65 6e 67 |  pGEM3 |   #leng|
|00001cb0| 74 68 20 32 38 36 37 20 | 20 20 23 63 68 65 63 6b |th 2867 |  #check|
|00001cc0| 73 75 6d 20 37 38 39 38 | 0d 53 45 51 55 45 4e 43 |sum 7898|.SEQUENC|
|00001cd0| 45 0d 0d 20 56 65 63 62 | 61 73 65 3a 50 67 65 6d |E.. Vecb|ase:Pgem|
|00001ce0| 33 20 20 4c 65 6e 67 74 | 68 3a 20 32 38 36 37 20 |3  Lengt|h: 2867 |
|00001cf0| 20 44 65 63 65 6d 62 65 | 72 20 31 31 2c 20 31 39 | Decembe|r 11, 19|
|00001d00| 38 39 20 20 31 30 3a 30 | 34 20 20 43 68 65 63 6b |89  10:0|4  Check|
|00001d10| 3a 20 37 38 39 38 20 20 | 2e 2e 00 00 00 00 00 00 |: 7898  |........|
|00001d20| 00 00 00 00 00 00 01 01 | 01 01 00 00 01 00 09 00 |........|........|
|00001d30| 2d 00 10 00 10 01 48 aa | 55 aa 55 aa 55 aa 55 dd |-.....H.|U.U.U.U.|
|00001d40| 6b 08 c2 06 a2 00 01 00 | 00 00 0a 00 0d 00 04 00 |k.......|........|
|00001d50| 1d 00 09 02 41 ab 54 ea | ff 00 03 00 c8 00 09 00 |....A.T.|........|
|00001d60| 11 00 00 00 00 00 00 00 | 04 00 2d 00 09 00 80 00 |........|..-.....|
|00001d70| 00 00 00 00 00 3f 80 00 | 00 00 00 00 fd 00 00 01 |.....?..|........|
|00001d80| 35 00 00 01 19 00 00 01 | 59 00 01 00 fc 00 09 00 |5.......|Y.......|
|00001d90| 00 00 00 00 00 00 be 00 | 80 00 00 00 00 06 31 34 |........|......14|
|00001da0| 35 2e 32 38 ad 3f b2 2d | 46 00 1c 60 04 2d 46 00 |5.28.?.-|F..`.-F.|
|00001db0| 1c 4c df 1c c0 4e 5e 20 | 5f de fc 00 14 4e d0 00 |.L...N^ |_....N..|
|00001dc0| 00 00 5e 40 80 e3 7a 40 | 80 65 44 00 00 6d 3c 00 |..^@..z@|.eD..m<.|
|00001dd0| 00 4c a2 00 01 71 00 00 | 00 00 10 02 64 00 5e 09 |.L...q..|....d.^.|
|00001de0| 40 40 81 2d 64 00 6e 00 | 01 00 6c 63 56 00 6d f0 |@@.-d.n.|..lcV.m.|
|00001df0| ac 40 81 27 a8 40 81 27 | 6e 00 6e 00 01 00 6c 63 |.@.'.@.'|n.n...lc|
|00001e00| 56 00 5e 09 40 40 81 2d | 64 00 6e 00 01 00 6c 63 |V.^.@@.-|d.n...lc|
|00001e10| 56 00 6d f0 ac 00 6d 40 | 81 27 a8 40 81 27 6e 00 |V.m...m@|.'.@.'n.|
|00001e20| 6e 00 01 00 6c 63 56 00 | 6d f0 ac 00 6d e7 0a 00 |n...lcV.|m...m...|
|00001e30| 6d 6f 6a 00 5e 85 e0 00 | 6d f7 30 00 5e 4c a2 00 |moj.^...|m.0.^L..|
|00001e40| 6d 6f fa 00 5e 85 e0 00 | 5e 80 34 00 00 f7 30 00 |mo..^...|^.4...0.|
|00001e50| 00 e3 22 40 80 65 44 00 | 5e 40 80 e3 7a 40 80 65 |.."@.eD.|^@..z@.e|
|00001e60| 44 00 00 6d 60 00 00 4c | a2 00 6e 00 01 00 6c 63 |D..m`..L|..n...lc|
|00001e70| 56 00 6e 00 01 00 6c 63 | 56 00 6d 6f fa 00 6d 00 |V.n...lc|V.mo..m.|
|00001e80| 6d f7 30 00 6d f7 30 00 | 6d 00 01 d3 56 00 5e 00 |m.0.m.0.|m...V.^.|
|00001e90| 6d e5 9e 00 5e 46 ba 00 | 00 01 26 00 6e 00 00 00 |m...^F..|..&.n...|
|00001ea0| 00 00 00 00 20 00 00 00 | 20 00 04 00 0c 00 00 00 |.... ...| .......|
|00001eb0| 0c 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001ec0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001ed0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001ee0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001ef0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001f00| 00 00 01 00 00 00 01 9a | 00 00 00 9a 00 00 00 46 |........|.......F|
|00001f10| 54 43 41 54 41 47 43 54 | 43 41 43 47 43 54 47 54 |TCATAGCT|CACGCTGT|
|00001f20| 41 47 47 54 41 54 43 54 | 43 41 47 54 54 43 47 47 |AGGTATCT|CAGTTCGG|
|00001f30| 05 70 47 45 4d 33 02 00 | 00 00 47 43 4b 63 47 43 |.pGEM3..|..GCKcGC|
|00001f40| 4b 20 00 00 00 00 00 00 | 00 00 00 00 15 26 00 00 |K ......|.....&..|
|00001f50| 00 00 47 43 4b 63 47 43 | 4b 20 00 00 00 00 00 00 |..GCKcGC|K ......|
|00001f60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001f70| 00 00 ab 83 7a 20 00 00 | 1e 31 00 00 01 e0 41 54 |....z ..|.1....AT|
|00001f80| 43 47 54 43 54 54 47 41 | 47 54 43 43 41 41 43 43 |CGTCTTGA|GTCCAACC|
|00001f90| 43 47 47 54 41 41 47 41 | 43 41 43 47 41 43 54 54 |CGGTAAGA|CACGACTT|
|00001fa0| 41 54 43 47 43 43 41 43 | 54 47 47 43 41 47 43 41 |ATCGCCAC|TGGCAGCA|
|00001fb0| 47 43 43 41 43 54 47 47 | 54 41 41 43 41 47 47 41 |GCCACTGG|TAACAGGA|
|00001fc0| 54 54 41 47 43 41 47 41 | 47 43 47 41 47 47 54 41 |TTAGCAGA|GCGAGGTA|
|00001fd0| 54 47 54 41 47 47 43 47 | 47 54 47 43 54 41 43 41 |TGTAGGCG|GTGCTACA|
|00001fe0| 47 41 47 54 54 43 54 54 | 47 41 41 47 54 47 47 54 |GAGTTCTT|GAAGTGGT|
|00001ff0| 47 47 43 43 54 41 41 43 | 54 41 43 47 47 43 54 41 |GGCCTAAC|TACGGCTA|
|00002000| 00 00 00 78 00 03 00 00 | 00 48 00 50 00 00 00 00 |...x....|.H.P....|
|00002010| 02 f0 02 80 ff dc ff ec | 02 f4 02 94 01 02 05 28 |........|.......(|
|00002020| 03 fc 00 01 00 00 00 48 | 00 50 00 00 00 00 02 f0 |.......H|.P......|
|00002030| 02 80 00 50 00 20 02 80 | 0c 80 00 18 00 01 01 01 |...P. ..|........|
|00002040| 00 00 00 01 27 0f 00 01 | 01 01 00 00 00 00 00 00 |....'...|........|
|00002050| 00 00 00 00 00 00 00 00 | 00 00 00 01 00 00 00 00 |........|........|
|00002060| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002070| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 1a |........|........|
|00002080| 01 a2 00 00 00 29 00 00 | 00 03 00 00 01 31 00 00 |.....)..|.....1..|
|00002090| 01 68 00 00 00 00 00 00 | 00 00 00 00 01 00 00 00 |.h......|........|
|000020a0| 01 9a 00 00 00 9a 00 00 | 00 46 00 58 19 50 07 b8 |........|.F.X.P..|
|000020b0| 00 00 00 1c 00 46 00 01 | 50 52 4e 54 00 00 00 12 |.....F..|PRNT....|
|000020c0| 73 74 61 74 00 00 00 1e | 00 01 ff ff 00 00 00 00 |stat....|........|
|000020d0| 00 00 00 00 44 89 ff ff | 00 00 00 7c 00 58 17 48 |....D...|...|.X.H|
|000020e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000020f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
+--------+-------------------------+-------------------------+--------+--------+